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KMID : 0545120120220111588
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 11 p.1588 ~ p.1590
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Characterization and cDNA Cloning of a Defensin-Like Peptide, Harmoniasin, from Harmonia axyridis
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Kim In-Woo
Lee Joon-Ha
Park Ha-Yan
Kwon Young-Nam
Yun Eun-Young
Nam Sung-Hee
Kim Seong-Ryul
Ahn Mi-Young
Hwang Jae-Sam
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Abstract
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We compared the mRNA expression profile of the Harmonia axyridis larvae that were either untreated or treated with LPS. The extracted mRNAs were subjected to ACP RTPCR analysis using a combination of arbitrary primers and oligo (dT) primer. Among the 47 DEGs differentially expressed, we identified a cDNA showing homology with defensin-like antibacterial peptide. The cDNA showed a putative 32-residue signal sequence and a 50-residue mature peptide named harmoniasin. We also investigated the antibacterial activity of the harmoniasin analog, which exhibited potent antibacterial activities against Gramnegative and -positive bacteria strains and it also evidenced no hemolytic activity.
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KEYWORD
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Harmonia axyridis, antimicrobial peptide, defensin, antibacterial activity
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